Proc. Natl. Acad. Sci. USA, 2003, Vol. 100, No. 25, pp 14840-14845.
Thermodynamic treatment of oligonucleotide duplex-simplex equilibria
Richard Owczarzy, Isard Dunietz, Mark A. Behlke, Irving M. Klotz and Joseph A. Walder
Thermodynamic formulations have been devised to obtain ΔGo values directly
from spectroscopic data at a fixed common temperature in nucleic acid
duplex-simplex melting curves. In addition, the dependence of melting on
salt concentration has been expressed in terms of a stepwise stoichiometric
representation, which leads to a specific equation for the partition of
the added sodium ions between the different oligonucleotide forms.
This article has been cited by other articles:
- Louw T.M., Whitney S.E., TerMaat J.R., Pienaar E., Viljoen H.J. (2011)
Oligonucleotide optimization for DNA synthesis.
AIChE J. 57, 1912-1918.
- Shao Y., Niu Z.J., Chen J.R., Zhang L.K. (2009)
Enhanced binding of a non-hydrogen bond ligand to DNA by introducing
an apurine/apyrimidine Site.
Chinese J. Chem. 27, 1117-1122.
- Chen Y.J., Huang X.H. (2009)
DNA sequencing by denaturation: Principle and thermodynamic simulations.
Anal. Biochem. 384, 170-179.
- Tan Z.J., Chen S.J. (2007)
RNA helix stability in mixed Na+/Mg2+ solution.
Biophys. J. 92, 3615-3632.
- Stellwagen E., Dong Q., Stellwagen N.C. (2007)
Quantitative analysis of monovalent counterion binding to random-sequence,
double-stranded DNA using the replacement ion method.
Biochemistry 46, 2050-2058.
- Tan Z.J., Chen S.J. (2006)
Nucleic acid helix stability: Effects of salt concentration, cation valence and
size, and chain length.
Biophys. J. 90, 1175-1190.
- Pfunder M., Frey J.E. (2005)
Dissociation analysis in polymerase chain reaction and 1x SSC buffer as a prerequisite for selection of 13mer microarray probe sets with uniform hybridization behavior.
Mol. Biotech. 29, 1-9.
- Onufriev A., Ullmann G.M. (2004)
Decomposing complex cooperative ligand binding into simple components: Connections between microscopic and macroscopic models.
J. Phys. Chem. B 108, 11157-11169.
Copyright 2013, Updated July 1, 2013, version 5.08.