Richard Owczarzy

Proc. Natl. Acad. Sci. USA, 2003, Vol. 100, No. 25, pp 14840-14845.

Thermodynamic treatment of oligonucleotide duplex-simplex equilibria

Richard Owczarzy, Isard Dunietz, Mark A. Behlke, Irving M. Klotz and Joseph A. Walder
Free reprint

Thermodynamic formulations have been devised to obtain ΔGo values directly from spectroscopic data at a fixed common temperature in nucleic acid duplex-simplex melting curves. In addition, the dependence of melting on salt concentration has been expressed in terms of a stepwise stoichiometric representation, which leads to a specific equation for the partition of the added sodium ions between the different oligonucleotide forms.

This article has been cited by other articles:
Copyright 2013, Updated July 1, 2013, version 5.08.